Short answers, pulled from the story.
Shakespeare's will was signed on the 25th of March 1616, just under a month before his death. The document had been drafted earlier, in January 1616, but was not signed until March, after Shakespeare made several significant alterations.
The reason Shakespeare left Anne his second-best bed remains disputed. Some scholars read it as a slight; others argue the second-best bed was the matrimonial bed and carried sentimental significance. Germaine Greer has suggested that the bulk of the estate passing to Susanna Hall reflected earlier marital agreements, not any coldness toward Anne.
The primary beneficiary was Shakespeare's daughter Susanna Hall, who inherited New Place, two houses on Henley Street, and various lands around Stratford. His daughter Judith received financial provisions totalling up to three hundred pounds under specific conditions, along with a silver-gilt bowl.
Shakespeare altered the will after it emerged that his new son-in-law Thomas Quiney had made a woman named Margaret Wheeler pregnant; she died in childbirth on the 15th of March 1616. Shakespeare struck out a provision for Quiney, inserted Judith's name in its place, and restructured her bequest to block Quiney from accessing the funds.
Shakespeare bequeathed each of his three Globe Theatre colleagues twenty-six shillings and eightpence to buy mourning rings. Some scholars, including Stanley Wells, have wondered whether this was also an implicit encouragement to publish a collected edition of his plays; within six years, Heminges and Condell did exactly that as editors of the First Folio.
The preamble states that Shakespeare hoped for salvation through "the only merits of Jesus Christ my Saviour", a formula identified as explicitly Anglican or Protestant. Catholic wills of the period typically invoked saints as intercessors, which Shakespeare's preamble does not do, though historians note the conventional nature of will preambles limits how far this can be taken as personal belief.