Short answers, pulled from the story.
Anne Gainsford was a lady-in-waiting to Anne Boleyn and later to Jane Seymour. She served in the household of Anne Boleyn before 1528 and continued her service after the Queen's execution in 1536.
In 1528 Anne Gainsford held a copy of William Tyndale's The Obedience of a Christian Man which was taken by her betrothed Sir George Zouche and delivered to Cardinal Thomas Wolsey. Anne Boleyn petitioned King Henry VIII to retrieve the text and persuaded him to read it, exposing the monarch to ideas that justified his break from Rome.
Anne Gainsford died before the 16th of July 1548 as confirmed by the last will and testament of her husband Sir George Zouche. The document from that date shows that George Zouche had remarried to a woman named Elleyn after Anne's death.
Anne Gainsford died before the 16th of July 1548 while George Wyatt was born around 1553 making it impossible for her to provide him with accounts. Modern research by Sylvia Barbara Soberton proved that Samuel Weller Singer's identification was wrong and that Wyatt likely spoke to another woman.
Anne Gainsford and Sir George Zouche established their principal home at Codnor Castle in Derbyshire after their marriage in 1533. Their lineage was recorded in the Derbyshire Visitation Pedigrees of 1569 and 1611 ensuring their place in the local gentry of Derbyshire.
The portrait identified as Anne Gainsford is a chalk drawing by Hans Holbein that remains a subject of debate among art historians. Many believe the portrait depicts Mary Zouche instead due to the name M. Souch in the top left corner of the artwork.